Carrillo C, Peñarrocha MA, Peñarrocha M, Vera-Sempere F, Peñarrocha D. Immunohistochemical study of Langerhans cells in periapical lesions: correlation with inflammatory cell infiltration and epithelial cell proliferation. Med Oral Patol Oral Cir Bucal. 2010 Mar 1;15 (2):e335-9.
Aim: The aim of this study is to determine the presence and distribution of Langerhans cells in periapical lesions,
and correlate this with inflammatory cell infiltration and epithelial cell proliferation. Material and Methods: Seventy
chronic dental periradicular lesions, obtained during periapical surgery from 70 patients, were included in
this study, including: 46 granulomas, 18 scar tissue and 6 periradicular cysts. Immunohistochemical staining was
performed using the following markers: CD3 to analyze the inflammatory infiltrate, CD1a to determine the presence
of Langerhans cells and Ki67 to analyze the epithelial cell proliferation. The CD1a immunostaining density was
established following Cincura (2007) criteria, being classified ranging from intense (3), moderate (2), discrete
(1) or no (0) immunostaining. CD3 and Ki67 staining was evaluated following the Liapatas et al. scale, as: 0) no
cells stained; 1) weak stain or few cells stained (11-25%); 2) moderate staining or some cells stained (26-75%);
3) intense staining or many cells stained (more than 76%). Results: Langerhans cells were found in 32.8% of the
periapical lesions being more intense in the epithelialized lesions. CD3 immunohistochemical staining was found
in all lesions, but with different values in relation to histological subtypes. Ki67 was positive in all epithelialized
lesions, although with a moderate staining. Conclusions: Langerhans cells appeared to be associated with
T-lymphocyte infiltration and the proliferative potential of the epithelial tissue in periapical lesions.